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The Direct Hemolytic Plaque Response to Hemocyanin in the Rabbit¹

From the Division of Immunochemistry and Allergy, McGill University Clinic, Royal Victoria Hospital, Montreal, Quebec, Canada

Abstract

Antibody-forming cells can be detected in the spleen and lymph nodes of rabbits immunized with keyhole limpet hemocyanin (KLH) by a direct plaque assay incorporating sheep red cells, coated with KLH by a chromic chloride method, as the target cell layer. After a single intravenous injection of associated KLH, direct plaque responses were observed in the spleen, with a peak at day 3, whereas no significant response was detectable in peripheral lymph nodes. When KLH in complete Freund's adjuvant was injected into hind footpads, the plaque response in regional lymph nodes was greater than that observed in the spleen. Numerically, the direct plaque response of rabbits to KLH was of the same order of magnitude as the response to bovine serum albumin reported by others.

Footnotes

¹ This work was supported by grants from the Medical Research Council of Canada, Quebec Medcal Research Council, and the Muscular Dystrophy Association of Canada.

² Medical Research Scholar, Medical Research Council of Canada.