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Antibody Weight Estimates at the Nanogram Level¹

From the Department of Medical Immunology, The Public Health Research Institute of the City of New York, Incorporated, New York, New York 10016

Abstract

A method is described for weight estimates of anti-dinitrophenyl antibodies in the nanogram range. The procedure is based on the interaction of the antibody preparation with a relatively large molar excess of the intensely iodinated dinitrophenylated dipeptide, DNP-L-lys,L-tyr,¹²⁵I. The hapten-antibody complex may be precipitated equally well by 45% saturated ammonium sulfate or a goat antiserum to rabbit globulins. Data are given to indicate some of the parameters governing the results, the effect of albumin and the methods of calculation.

Footnotes

¹ Support for this investigation was received, in part, from the National Science Foundation, Grant GB-1120; the American Cancer Society, Inc., Grant T-257; the National Institute of Allergy and Infectious Diseases of the United States Public Health Service, Grant AI-98710; and from the Office of The Surgeon General, Department of the Army, under the auspices of the Commission on Immunization of the Armed Forces Epidemiological Board, Contract DADA17-68-C-8091.