HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Siraganian, R. P. Articles by Osler, A. G. Search for Related Content PubMed Articles by Siraganian, R. P. Articles by Osler, A. G.

Destruction of Rabbit Platelets in the Allergic Response of Sensitized Leukocytes¹

I. Demonstration of a Fluid Phase Intermediate

From the Department of Medical Immunology, The Public Health Research Institute of the City of New York, Inc., New York, New York 10016

Abstract

The interaction of antigen with sensitized rabbit leukocytes results in histamine release and is cytotoxic for platelets in the reaction mixtures. The destruction of the platelets requires no exogenous serum factors and is proportional to the number of sensitized leukocytes in the reaction mixtures. Destruction of the platelets follows the release of one or more soluble intermediate(s) when the sensitized leukocytes react with antigen. The release of this factor(s) from leukocytes requires Ca²⁺ (optimal at 3 x 10^-4 M) and is inhibited by Mg²⁺. The action of this factor on platelets is temperature sensitive and is essentially complete in 5 min. The activity required for platelet destruction is also mediated by Ca²⁺ and is not inhibited by Mg²⁺. These observations suggest that the histamine release from sensitized rabbit leukocytes is associated with extracellular appearance of other, as yet unidentified substances whose activity is both temperature and calcium dependent.

Footnotes

¹ Support for this investigation was received, in part, from the National Science Foundation, Grant GB-1120; the American Cancer Society, Inc., Grant T-257; the National Institute of Allergy and Infectious Diseases of the United States Public Health Service, Grant AI-98710; and the Office of The Surgeon General, Department of the Army, under the auspices of the Commission on Immunization of the Armed Forces Epidemiological Board, Contract DADA1768-C-8091.

This article has been cited by other articles:

				G. Montrucchio, G. Alloatti, and G. Camussi Role of Platelet-Activating Factor in Cardiovascular Pathophysiology Physiol Rev, October 1, 2000; 80(4): 1669 - 1699. [Abstract] [Full Text] [PDF]

				S. A. Carlson, T. K. Chatterjee, and R. A. Fisher The Third Intracellular Domain of the Platelet-activating Factor Receptor Is a Critical Determinant in Receptor Coupling to Phosphoinositide Phospholipase C-activating G Proteins. STUDIES USING INTRACELLULAR DOMAIN MINIGENES AND RECEPTOR CHIMERAS J. Biol. Chem., September 20, 1996; 271(38): 23146 - 23153. [Abstract] [Full Text] [PDF]

				H. W. Mueller, C. A. Haught, J. M. McNatt, K. Cui, S. J. Gaskell, D. A. Johnston, and J. T. Willerson Measurement of Platelet-Activating Factor in a Canine Model of Coronary Thrombosis and in Endarterectomy Samples From Patients With Advanced Coronary Artery Disease Circ. Res., July 1, 1995; 77(1): 54 - 63. [Abstract] [Full Text]