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Antibodies Produced After Immunization with Substituted Meta-Dinitrobenzenes

Production of ₁ and ₂ Classes of Antibody Including Complement-Dependent Antibodies

From the Laboratory of Bacterial products, Division of Biologics Standards, National Institutes of Health, United States Department of Health, Education and Welfare, Bethesda, Maryland 20014

Abstract

The serum antibodies induced in guinea pigs after immunization with 5 µmols of a series of substituted meta-dinitrobenzenes in Freund's adjuvant were investigated. The frequency of induction of precipitating and anaphylactic antibodies was directly related to the chemical reactivity of the immunizing material; no such relation was found for induction of hemagglutinating antibodies. The most reactive chemicals and one preformed conjugate (DNP₃₇BSA) induced antibodies in more than 80% of the animals but the types of antibody varied. 2,4-Dinitrofluorobenzene induced high titers of anaphylactic antibodies, high levels of ₁ immunoglobulins and an antibody of ₁ mobility whose precipitation was complement-dependent. 3,5-Dinitrobenzoyl chloride induced high titers of hemagglutinating antibody, high levels of ₂ immunoglobulins and a ₂-precipitating antibody whose precipitation was complement-independent. The conjugate induced equal titers of anaphylactic and hemagglutinating antibodies and equal amounts of ₁ and ₂ precipitins. The antibodies induced were extensively cross-reactive, in contrast to the great specificity shown earlier for the delayed response to these chemicals.