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Molecular Immunogenicity of Bacterial Lipopolysaccharide Antigens: Establishing a Quantitative System

United States Department of Health, Education, and Welfare, Public Health Service, National Institutes of Health, National Institute of Allergy and Infectious Diseases, Rocky Mountain Laboratory, Hamilton, Montana 59840

Abstract

Primary injection of lipopolysaccharide (LPS) from Escherichia coli sensitizes mice for a heightened immunologic response to a constant secondary dose of the same LPS. The lower dose limit for primary sensitization is a few tens of molecules and the height of the secondary response is proportional to the size of the dose of LPS over a 10¹²-fold range. Linear dose-response curves can also be established for the secondary injection of LPS, but over a much smaller range. A minimum of 10 to 14 days is required between injections of LPS to obtain consistently maximum titers, and the capacity of mice to give a secondary response is maintained for at least 12 weeks following the primary injection of LPS. The results suggest that this system provides a sufficiently sensitive and reproducible test for continuing studies on the molecular immunogenicity of LPS antigens.

Footnotes

¹ Present address: Department of Microbiology, University of Montana, Missoula, Montana 59801.