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The Journal of Immunology, 1971, 106: 467-472.
Copyright © 1971 by The American Association of Immunologists, Inc.

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The Fifth Component of the Guinea Pig Complement System

I. Purification and Characterization1

Charles T. Cook, Hyun S. Shin, Manfred M. Mayer and Karen A. Laudenslayer

From the Department of Microbiology, The Johns Hopkins University School of Medicine, Baltimore, Maryland

Abstract

A procedure is described for isolation of guinea pig C5. The product has a sedimentation coefficient of 7.8S and a molecular weight of about 180,000. It is free of the other eight complement components and no substantial non-complement contamination is present, as indicated by immunoelectrophoretic tests.

Footnotes

1 This work was supported by National Science Foundation Grant GB-7406X, United States Public Health Service Grant 5-RO1-AI-02566-10, United States Public Health Service Training Grant 5TO1-AI-00282-05 and a contract with the Office of Naval Research ONR 248(60).







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