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Isolation of Rabbit Anti-Rhesus Lymphocyte Antibody Using a Specific Immunoadsorbent^1,2,

From The Oregon Regional Primate Research Center, Beaverton, Oregon 97005

Abstract

This report describes a rapid, one-step method of purifying both anti-rhesus and anti-human lymphocyte antibodies using a specific immunoadsorbent. The immunoadsorbent was prepared by chemical conjugation of rhesus lymphocyte stroma to Sephadex G-100. Specifically purified antilymphocyte antibody eluted from the immunoadsorbent represented 6% to 9% of the total protein applied to the column. The antibody preparations showed a 10- to 15-fold increase in specific activity (leukoagglutinins/milligram IgG).

Footnotes

¹ This work was supported by United States Public Health Service Grants AI-00139 and AI-06274.

² Some of the results reported in this paper were presented at the annual meeting of the American Association of Immunologists, Atlantic City, New Jersey, April 1970.

³ Submitted in partial fulfillment of the requirements for a doctoral degree.