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The Journal of Immunology, 1970, 105: 262-264.
Copyright © 1970 by The American Association of Immunologists, Inc.

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Some Characteristics of Anti-Dinitrophenyl Antibody of the Gray Snapper1

Warren J. Russell, Edward W. Voss, Jr. and M. Michael Sigel

From the Department of Microbiology, University of Miami School of Medicine, Miami, Florida 33152, the Variety Children's Research Foundation, Miami, Florida, the Lerner Marine Laboratory, Bimini, Bahamas, and the Department of Microbiology, University of Illinois, Urbana, Illinois 61801

Abstract

Gray snappers were immunized with dinitrophenylated hemocyanin and purified anti- bodies to the haptenic determinant (2,4-dinitrophenol) were obtained from two serum pools by by immune absorption. Examination of these antibodies revealed a single high molecular weight protein with an apparent valence of 5 and a relatively low binding constant.

Footnotes

1 This work was supported by Public Health Service Research Grants AI-05758 and AI-08288 from the National Institute of Allergy and Infectious Diseases.







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