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The Journal of Immunology, 1970, 105: 154-161.
Copyright © 1970 by The American Association of Immunologists, Inc.
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Immunoglobulin Classes of Antibody-Forming Cells in Mice

IV. The Incorporation of Tritiated Thymidine Into IgM and {gamma}1 Plaque-Forming Cells During the Primary Immune Response1

Albert A. Nordin2, Humberto Cosenza and Stewart Sell3

From the Department of Microbiology, Lobund Laboratory, University of Notre Dame, Notre Dame, Indiana 46556, and the Department of Pathology, University of Pittsburgh, School of Medicine, Pittsburgh, Pennsylvania 15213

Abstract

The incorporation of tritiated thymidine (3HT) into hemolysin-in-agar plaque-forming cells (PFC) was determined for direct (IgM) and indirect ({gamma}1) PFC following a single optimal injection of sheep red blood cells (SRC) into mice. After immunization with SRC, 3HT was injected into mice at various time intervals. The PFC of all mice tested were assayed at 5.5 days after immunization because {gamma}1 PFC are not detectable in significant numbers until this time. The percentage of PFC labeled and the number of grains/labeled cell indicated that the onset, duration and extent of incorporation of 3HT were the same for both types of PFC. The potential {gamma}1 PFC appear to be formed at the same time as the IgM PFC, but {gamma}1 PFC do not synthesize or release {gamma}1 antibody until 5 to 6 days after immunization, when proliferation of PFC has essentially stopped. IgM PFC appear 2 days after immunization and appear to synthesize DNA, divide and produce antibody simultaneously.

Footnotes

1 This work was supported by USPHS Grants Al-07659 and Al-07712.

2 Recipient of Career Development Award 1K 2-Al-37562.

3 Recipient of Career Development Award 1K 3-Al-23308.






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