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Studies on IgA

II. Isolation of IgA from Small Volumes of Human Serum by Immunoabsorption¹

From the Clinical Immunology Section, Hektoen Institute of Medical Research of the Cook County Hospital, and the Department of Medicine, University of Illinois College of Medicine, Chicago, Illinois

Abstract

Bromoacetyl cellulose was used to prepare a monospecific anti-IgA immunoabsorbent for the one-step isolation of IgA from small volumes of human serum. Two features were essential to this procedure. a) Anti-IgA globulin was freed of inert globulin by immunoabsorption and was used for coupling to bromoacetyl cellulose. The binding capacity of this immunoabsorbent was 15 mg IgA/g absorbent (using whole human serum). b) 3 M urea wash solution was used to minimize contamination of the eluted IgA by nonspecifically absorbed serum proteins. The IgA obtained by this method was 98.5% pure; it retained biologic activity and showed the electrophoretic pattern of IgA in serum.

Footnotes

¹ These studies were supported by Research Contract NIH 69-2092 (Principal Investigator, Truman O. Anderson), Vaccine Development Branch, National Institute of Allergy and Infectious Diseases, National Institutes of Health.

² Present address: Department of Biochemistry, Presbyterian-St. Luke's Hospital, Chicago, Illinois 60612.