HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Cosenza, H. Articles by Nordin, A. A. Search for Related Content PubMed Articles by Cosenza, H. Articles by Nordin, A. A.

Immunoglobulin Classes of Antibody-Forming Cells in Mice

III. Immunoglobulin Antibody Restriction of Plaque-Forming Cells Demonstrated by the Double Immunofluorescent Technique^1,2,

From the Department of Microbiology, Lobund Laboratory, University of Notre Dame, Notre Dame, Indiana 46556

Abstract

The biosynthetic potential of IgM and IgG plaque-forming cells in the spleens of mice during the first 8 days after immunization with sheep erythrocytes was examined. The double immunofluorescence technique was used to detect cells synthesizing both IgM and IgG hemolysins simultaneously. A total of 5944 plaque-forming cells (PFC) were examined: 2329 were IgG hemolysin producers, 3585 were IgM hemolysin producers, and only 30 could be classified as double (IgM-IgG) antibody producers. The proportion of double producers never exceeded 1.2% and it was usually below 1%.

It is concluded that the single antibody-producing cell is phenotypically restricted to the synthesis of one immunoglobulin antibody class at a time. The percentage (0.79%) of double IgM-IgG producers that was found at the time when IgM PFC were decreasing and IgG PFC were increasing in number is too low to suggest that the rapid rise of IgG PFC was due to the majority of the IgM PFC switching to the production of IgG hemolysin.

Footnotes

This study was supported by United States Public Health Service Grant AI-07659.

² Submitted to the Graduate School of the University of Notre Dame in partial fulfillment of the requirements for the degree of Doctor of Philosophy.

³ Supported by Marion County Cancer Society.

⁴ Recipient of National Institutes of Health Career Development Award 1K 3-AI-37562.

This article has been cited by other articles:

				D. Kabat Gene Selection in Hemoglobin and in Antibody-Synthesizing Cells Science, January 14, 1972; 175(4018): 134 - 140. [Abstract] [PDF]