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Biologic Activities of Aggregated Immunoglobulin E¹

From the Children's Asthma Research Institute and Hospital, Denver, Colorado, and the Institute of Biochemistry, University of Uppsala, Uppsala, Sweden

Abstract

Nonspecifically aggregated E myeloma protein induced erythema-wheal reactions in human and monkey skin but not in guinea pig skin. The minimum doses of the aggregated E required to induce the reactions in the normal human and monkey skin were 1 ng N and 10 ng N, respectively. Monomer protein did not give such a skin reaction in any of the three animal species. Evidence was presented that combination of the aggregated E with target cells, to which reaginic antibodies attach upon sensitization, is essential for induction of the skin reactions in the primates. Aggregated G induced erythema-wheal reactions in all of the three animal species studied, but the minimal reactive dose of the aggregated G was 200 to 500 times more than the dose of aggregated E. E myeloma proteins sensitized monkey but not guinea pig skin for reversed type passive cutaneous anaphylaxis. In contrast, normal G that had skin-sensitizing activity in the guinea pig failed to sensitize monkey skin.

Aggregated E, which had skin reactivity in the primates, did not fix complement. Lack of complement fixation by the aggregated E was confirmed by C1a and C3 fixation tests. Failure of aggregated E to fix complement indicated that neither complement nor anaphylatoxin was involved in the mechanisms of reaginic hypersensitivity reaction by E systems.

Footnotes

¹ This work was supported mainly by Grant AI-04985 from the United States Public Health Service and in part by Grant GB-8370 from the National Science Foundation. This paper was presented before the 53rd annual meeting of the American Association of Immunologists, April 1969, Atlantic City, New Jersey.