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Rapid Detection of Australia Antigen by Counterimmunoelectrophoresis¹

From the Departments of Medicine and Microbiology, College of Physicians and Surgeons, Columbia University, New York, New York

Abstract

This report describes the detection of Australia antigen by counterelectrophoresis in agar gel. With this technique small amounts of antigen with relatively rapid electrophoretic mobility can be detected by specific precipitation with antibody in 1 hr (1, 2). In addition to its speed the technique is at least 10 times as sensitive as the Ouchterlony double diffusion method.

Materials and Methods. Sera containing Australia antigen were obtained from patients with acute viral hepatitis. Specific antisera were from hemophiliacs who had received numerous blood transfusions and were thus repeatedly exposed to hepatitis virus. The standard sera containing Australia antigen used in this laboratory, as well as the homologous antisera, have been shown to have the same specificities as those employed by Blumberg (3) and Prince (4).

Counterelectrophoresis is carried out on Kodak projection slides (3.25 x 4 in) covered with 10 ml of 0.85% Agarose in Veronal buffer, 0.05 M, pH 8.2.

Footnotes

This work was supported by Grants AI-08890 and AI-03168 from the National Institutes of Health, United States Public Health Service.

This article has been cited by other articles:

				G. N. Vyas, K. R. Rao, and A. B. Ibrahim Australia Antigen (Hepatitis B Antigen): A Conformational Antigen Dependent on Disulfide Bonds Science, December 22, 1972; 178(4067): 1300 - 1301. [Abstract] [PDF]

				A. M. Prince and K. Burke Serum Hepatitis Antigen (SH): Rapid Detection by High Voltage Immunoelectroosmophoresis Science, August 7, 1970; 169(3945): 593 - 595. [Abstract] [PDF]