HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via Google Scholar Google Scholar Articles by Thunold, S. Articles by Milgrom, F. Search for Related Content PubMed Articles by Thunold, S. Articles by Milgrom, F.

Reactions in Agarose Gel between Clq and Aggregated Globulin¹

Department of Microbiology, State University of New York at Buffalo, School of Medicine, Buffalo, New York 14214

Abstract

The reaction of normal human and animal sera with heat-aggregated serum globulin (FII) was studied by means of double diffusion precipitation tests in agarose gel. Best results were obtained using agarose dissolved in a low molar salt solution (0.075 M NaCl). Precipitation lines were obtained, and in some instances improved, in the presence of EDTA. The double diffusion tests in agarose gel could be employed as a simple and convenient analytic tool for identification of the precipitin as the Clq component of complement.

The reaction of Clq of human and animal origin was stronger with aggregated rat FII than with aggregated human FII. No reactions were obtained with bovine, sheep or rabbit FII. The anticomplementary activity of FII preparations in a hemolytic system paralleled their activity in the precipitation tests.

Eluates containing Clq were prepared from precipitates formed in the reaction between human serum and rat FII in the presence of EDTA. Antisera specific for human Clq were produced in rats by injection of such precipitates.

Footnotes

This study was partially supported by United States Public Health Service Research Grant CA-02357 from the National Cancer Institute.

² Recipient of an Eleanor Roosevelt International Cancer Fellowship of the American Cancer Society awarded by the International Union Against Cancer. Present address: The Gade Institute, Department of Pathology, University of Bergen, 5000 Bergen, Norway.