| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
Immunobiology Branch, Armed Forces Institute of Pathology, Washington, D. C. 20305
Abstract
Lysosomal granules from rabbit neutrophilic leukocytes contain an enzyme that cleaves the fifth component of human complement (C5) into chemotactically active fragments. The enzyme cleaves C5, but not the third component of complement, into chemotactically active fragments of variable molecular weights, depending upon conditions of incubation. One fragment consistently behaves in ultracentrifugation and gel filtration similar to the reference standard cytochrome c. The amount of chemotactic activity generated by interaction of the lysosomal granule lysate and C5 is a function of concentrations of C5 and lysate, as well as duration of incubation.
The C5-cleaving enzyme of neutrophil lysosomal granules has a neutral pH optimum for activity. Based upon gel filtration, it has an estimated molecular weight of 35,000, is inhibited by esters bearing basic amino acids, is susceptible to inhibition by 
-amino caproic acid and in varying degree is susceptible to action of soybean trypsin inhibitor and EDTA.
The presence in the neutrophil of an enzyme capable of cleaving C5 into chemotactically active fragments may reflect the potential for the neutrophil to exacerbate in a non-immunologic manner the acute inflammatory process once it is underway.
Footnotes
This work was supported in part by Grant AI-07291-AIA from the National Institutes of Health, under the auspices of Universities Associated for Research and Education in Pathology, Inc. The opinions or assertions contained herein are the private views of the authors and are not to be construed as official or as reflecting the views of the Department of the Army or the Department of Defense.
2 Supported by the Ohio Division of the American Cancer Society.
This article has been cited by other articles:
|
|
 |
|
  Y. Fukuoka, H.-Z. Xia, L. B. Sanchez-Munoz, A. L. Dellinger, L. Escribano, and L. B. Schwartz Generation of Anaphylatoxins by Human {beta}-Tryptase from C3, C4, and C5 J. Immunol., May 1, 2008; 180(9): 6307 - 6316. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Garantziotis, J. W. Hollingsworth, R. B. Ghanayem, S. Timberlake, L. Zhuo, K. Kimata, and D. A. Schwartz Inter-{alpha}-Trypsin Inhibitor Attenuates Complement Activation and Complement-Induced Lung Injury J. Immunol., September 15, 2007; 179(6): 4187 - 4192. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Mihai, M. T. Chiriac, K. Takahashi, J. M. Thurman, V. M. Holers, D. Zillikens, M. Botto, and C. Sitaru The Alternative Pathway of Complement Activation Is Critical for Blister Induction in Experimental Epidermolysis Bullosa Acquisita J. Immunol., May 15, 2007; 178(10): 6514 - 6521. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. M. Bless, M. Huber-Lang, R.-F. Guo, R. L. Warner, H. Schmal, B. J. Czermak, T. P. Shanley, L. D. Crouch, A. B. Lentsch, V. Sarma, et al. Role of CC Chemokines (Macrophage Inflammatory Protein-1{beta}, Monocyte Chemoattractant Protein-1, RANTES) in Acute Lung Injury in Rats J. Immunol., March 1, 2000; 164(5): 2650 - 2659. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. S. Mulligan, A. B. Lentsch, M. Huber-Lang, R.-F. Guo, V. Sarma, C. D. Wright, T. R. Ulich, and P. A. Ward Anti-Inflammatory Effects of Mutant Forms of Secretory Leukocyte Protease Inhibitor Am. J. Pathol., March 1, 2000; 156(3): 1033 - 1039. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. S. Gipson, N. M. Bless, T. P. Shanley, L. D. Crouch, M. R. Bleavins, E. M. Younkin, V. Sarma, D. F. Gibbs, W. Tefera, P. C. McConnell, et al. Regulatory Effects of Endogenous Protease Inhibitors in Acute Lung Inflammatory Injury J. Immunol., March 15, 1999; 162(6): 3653 - 3662. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. J. Czermak, V. Sarma, N. M. Bless, H. Schmal, H. P. Friedl, and P. A. Ward In Vitro and In Vivo Dependency of Chemokine Generation on C5a and TNF-{alpha} J. Immunol., February 15, 1999; 162(4): 2321 - 2325. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. J. Heyman and J. E. Rinaldo Multiple System Organ Failure in the Adult Respiratory Distress Syndrome J Intensive Care Med, September 1, 1989; 4(5): 192 - 200. [Abstract] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
