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Synthesis of Secretory IgA in the Rabbit

II. Production of Alpha, Light and T Chains by in Vitro Cultures of Mammary Tissue

From the Laboratories of Clinical Investigation, Microbial Immunity, and Immunology, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Md. 20014

Abstract

Rabbit colostral IgA internally labeled with ¹⁴C has been isolated from culture fluids of short term cultures of mammary tissue obtained from three rabbits. Alpha, light, and T chains of intact or extensively reduced and alkylated IgA were separated by Sephadex G-200 chromatography in 5 M guanidine, and by polyacrylamide disc electrophoresis. The results indicate that all three chains are synthesized locally, and that ¹⁴C lysine and isoleucine are incorporated into , light and T chains in a ratio of approximately 4:2:1. This ratio is in reasonably close agreement with the mass ratio of the chains of an IgA molecule with 4, 4 light, and 2 T chains. The data indicate that local synthesis can account for all of the IgA found in rabbit colostrum.

Footnotes

¹ Present address: Department of Pediatrics, University of Alabama Medical Center, 1919 7th Avenue, South, Birmingham, Alabama 35233.

² The additional polypeptide chain found in rabbit secretory IgA has been designated T chain by Cebra and Small (1) and what is apparently a homologous chain in human secretory IgA has been called secretory piece by Tomasi and associates (3). In this paper T chain is used in reference to rabbit material and secretory piece to human material.