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Properties of Purified Mouse Homocytotropic Antibody¹

From the Department of Microbiology, University of Illinois, Urbana, Illinois

Abstract

Purified anti-DNP antibody was obtained from a pool of hyperimmune mouse sera. Purity was verified by radioimmunoelectrophoresis, disc gel electrophoresis and sedimentation equilibrium studies. A molecular weight of 141,500 was determined by the method of Yphantis. A corrected valence of 2 and an average intrinsic association constant of 6 x 10⁵ liters/mol was measured by equilibrium dialysis.

The purified antibody elicited a positive PCA reaction in mice only after a 3-hr incubation period. The length of the incubation period, the electrophoretic mobility and the heat stability at 56°C indicated that this antibody belonged to the 7S ₁ class of mouse immunoglobulin.

Footnotes

This work was supported by Grant AI-08288 from the National Institute of Allergy and Infectious Diseases.