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Lymphoid Precursors: Thymus Independent Antibody Production^1,2,

From the Naval Radiological Defense Laboratory, San Francisco, California 94135³, and the Department of Genetics, Stanford University School of Medicine, Stanford, California 94305^,4

Abstract

Thymectomized and nonoperated adult mice were lethally irradiated and given liver cells from embryos of various ages. The resultant chimeras were hyperimmunized with sheep erythrocytes, and cell suspensions from their spleens were used in a hemolytic plaque assay employing specific anti-allotype antisera as developing agents in order to distinguish between cells producing host and donor-type G_2a (7S) antibodies. It was found that lymphiod stem cells taken from embryos as early as the 10th day of gestation (i.e., before the appearance of the thymic rudiment) were able to produce G_2a anti-SRBC antibodies in thymectomized hosts. The number of donortype "developed" plaques found in the spleens of thymectomized hosts was approximately one-tenth that found in their nonoperated controls.

Sensitization of the hosts to SRBC before irradiation and the transfer of embryonic liver cells did not increase the number of donor-type PFC.

Footnotes

This work was supported by funds from Bureau of Medicine and Surgery, United States Navy, and by Grants CA-04681 and GM-12075 from the National Institutes of Health.

² The research was conducted according to the principles enunciated in the ‘Guide for Laboratory Animal Facilities and Care’ prepared by the National Academy of Sciences, National Research Council.

³ M. L. Tyan. Present address: Stanford Research Institute, Menlo Park, Calif. 94025.

⁴ L. A. Herzenburg and P. R. Gibbs.