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Insoluble Immunoadsorbents Containing Anti-IgE: Removal of Reaginic Activity and Subsequent Elution¹

From the Departments of Medicine and Microbiology, State University of New York at Buffalo and the Allergy Research Laboratory, Buffalo General Hospital, 100 High Street, Buffalo, New York

Abstract

Reaginic antibody plays a significant role in immediate hypersensitivity of man. Ishizaka et al. (1) have reported that the human atopic reagin is primarily IgE protein, and these investigators have prepared rabbit (1) and guinea pig antisera (2) to human IgE by immunization with a purified reagin-rich fraction from ragweed sensitive patients. Johansson et al. (3) produced antiserum to human IgND (IgE), employing for immunization the protein from a patient with IgND (IgE) myeloma. Stanworth et al. (4) were able to block a Prausnitz-Küstner (PK) reaction with the Fc fragment of the IgND (IgE) myeloma protein, indicating that the reaginic antibody is related to the IgND (IgE). The antisera produced by Ishizaka and Johansson were shown to have identical serologic properties (5).

Through the courtesy of Dr. M. Ogawa, we obtained a small amount of plasma from a patient with IgE myeloma (for case description see (6)). With this material we were able to prepare rabbit antiserum to human IgE.

Footnotes

This work was supported by research Grant AI-01313, National Institutes of Health, Bethesda, Maryland.