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Immunoglobulins of the Rhesus Monkey (Macaca Mulatta)

IV. Characteristics of the primary and secondary responses of the rhesus monkey to heterologous serum protein antigens¹

From the Departments of Microbiology and Medicins, Northwestern University School of Medicine, Chicago, Illinois

Abstract

The immune response of the rhesus monkey (Macaca mulatta) to bovine serum albumin (BSA) and bovine globulin (BGG) has been examined by simultaneous injection of the two antigens isotopically labeled with ¹²⁵I and ¹³¹I, respectively. The kinetics of both antigen elimination and antibody synthesis varied widely within the group of animals studied. BSA was exponentially eliminated from the circulation with a half-life of 7.5 ± 0.7 days and BGG with a half-life of 2.7 ± 0.6 days. Circulating anti-BSA antibody was initially detectable 17.0 ± 3.2 days after injection of antigen in the primary response and 10.2 ± 3.8 days after injection of antigen in the secondary response. Anti-BGG antibody was first detected at 16.4 ± 5.3 days in the primary response and at 10.3 ± 2.1 days in the secondary response. A frequent lack of correlation was observed between anti-BSA hemagglutination titers and ammonium sulfate binding measurements even though only IgG-type antibody was demonstrable in most instances. This was interpreted to indicate an intra-class heterogeneity of IgG antibody with respect to hemagglutinating activity. Primary anti-BSA and anti-BGG antibody responses of both the IgG and mixed IgG-IgM types were detected. In the latter type of response, IgG and IgM antibody appeared simultaneously. IgM-type antibody was observed for only the first 6 to 8 days of the response, only IgG antibody being present thereafter. In the secondary response to both antigens, all monkeys produced IgG antibody alone, except for one animal in which an immunoglobulin thought to be IgA exhibited anti-BSA binding activity for a period of 9 days.

Footnotes

This work was supported in part by the Ernest S. Bazley Asthma Research Fund to Chicago Wesley Memorial Hospital, Chicago, Ill.

² University Post-Doctoral Scholar in the Medical Scientist Training Program, United States Public Health Service, under Traineeship 5-T5-GM-01671-03.

³ Ernest S. Bazley Professor of Alelrgy and Immunology.