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Factors in Delayed Sensitivity: Lymphocyte and Macrophage Cytotoxins in the Tuberculin Reaction¹

From the Department of Microbiology, School of Medicine, University of Washington, Seattle, Washington 98105

Abstract

Highly purified lymph node and spleen lymphocytes and lung macrophages obtained from normal and tuberculin sensitive guinea pigs were cultured in vitro with tuberculin purified protein derivative (PPD). After 24 hr of incubation, the cell-free culture supernatants were tested for a) mouse L cell cytotoxicity, b) inhibition of macrophage migration, and c) capacity to elicit skin reaction in the skin of non-sensitized guinea pigs. Purified lymphocytes from tuberculin-sensitive guinea pig cultured with PPD yielded culture supernatants which inhibited the growth of L cells. In contrast, purified macrophages from both normal and sensitive animals cultured with PPD released a factor(s) which was cytotoxic for L cells. Macrophage and lymphocyte cytotoxins could not be distinguished by gel filtration, antibody neutralization, heat sensitivity or by the biologic assays employed.

Footnotes

Supported in part by Graduate Research Training Grant CA-5040 from the National Cancer Institute.

² Graduate Research Trainee (CA-5040, National Cancer Institute).