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Distribution Analysis of Brain-Localized Radioantibodies after Zonal Ultracentrifugation in a Sucrose Density-Gradient¹

From the Department of Microbiology and Immunology, Duke University Medical Center, Durham, North Carolina 27706

Abstract

Through the process of brain-localization in vivo after intravenous injection, of homogenization and zonal centrifugation in a sucrose density gradient and of multi-compartment distribution analysis, an antibrain radioantibody, paired with normal radioiodoglobulin, was shown to have localization properties in rat brain. Of the brain-localized immunoglobulin, 85% was shown to be antibody that had a distribution profile with four main density peaks and several lesser ones. In contrast, the 15% that was shown to be normal globulin had a profile with only one major density peak, that of the soluble protein compartment.

Footnotes

This study was supported by AEC Contract AT-(40-1)-3195.