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Immunosuppression by Leukemia Viruses

III. Adoptive transfer of antibody-forming cells to friend disease virus-infected mice¹

From the Departments of Microbiology, Temple University School of Medicine and Albert Einstein Medical Center, Philadelphia, Pennsylvania

Abstract

Specific antibody-forming cells contained in spleen cell suspensions from normal immunized donor mice were adoptively transferred to both normal and Friend disease virus-infected recipient mice. There was little or no suppression with time in the number of the transferred antibody-forming cells in infected animals as compared to controls, under conditions in which there was a marked suppression of antibody-forming cells in mice directly challenged with antigen.

A study of the cytokinetics of the antibody plaque response in infected and control recipients indicated that the largest number of plaque-forming cells (PFC) could be determined in recipient spleens between 1 and 3 days after cell transfer. However, there were significant numbers of PFC in recipient spleens 6 and 9 days after transfer.

Infection of recipient mice simultaneously or 3 days before cell transfer did not result in an inhibition of the expected number of PFC. In most instances there was a 60% to 70% increase in the number of plaque-forming cells in spleens of mice infected the same day as cell transfer during the subsequent 3 days.

Recipient mice infected 8 days before cell transfer generally had 25% to 40% fewer antibody-forming cells in their spleens on the peak day after cell transfer, as compared to control recipients. However, there were almost normal numbers of antibody- forming cells 6 to 9 days after transfer, as compared to controls and the other groups of infected recipients.

The results of these experiments have been interpreted as indicating that the immunosuppressive effects of a murine leukemia virus such as Friend disease virus do not occur at the level of mature antibody-forming cells.

Footnotes

This work was supported in part by an Ethel A. Shaffer Memorial Grant from the American Cancer Society (T328C), an Institutional Grant to Temple University from the American Cancer Society (IN88A), and the National Science Foundation (GB7719).