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Identification of an Antigen and Specific Antibody in the Sera of Chickens Infected with Plasmodium Gallinaceum¹

From the Department of Veterinary Pathology and Hygiene and the Department of Microbiology, University of Illinois, Urbana, Illinois 61803

Abstract

A study based on a double diffusion gel assay of pre- and post-infection sera with a standard antisoluble antigen serum pool demonstrated antigens in chicken sera during the acute phase of infection with Plasmodium gallinaceum. In a single chicken experiment, an antigen and specific antibody were demonstrated in one individual during the acute and convalescent phases of infection, respectively.

The antigen, designated soluble antigen one (SA 1), was a specific molecular entity associated with the descending portion of the macroglobulin peak. After Sephadex G-200 column chromatography, the purity of the antigen was ascertained by immuno- and disc-electrophoresis and analytical ultracentrifugation. The molecular weight of SA 1 was estimated at 500,000 to 700,000 on the basis of molecular sieve chromatography, ultracentrifugation and observations with disc-electrophoresis.

Specific antibody which reacted with SA 1 was precipitated from convalescent serum with 12.5% sodium sulfate and then eluted from DEAE in the 0.02 M and 0.10 M phosphate fractions. This antibody was identified as IgG.

Footnotes

This study was supported by National Institutes of Health Research Grant HE 10609, and in part by the Mark L. Morris Animal Research Foundation. Technical assistance of Mrs. Laurel Karr is gratefully acknowledged. This paper was presented in part at the 17th annual meeting of the American Society of Tropical Medicine and Hygiene, Atlanta, Georgia, November 1, 1968.

² This article derives in part from the theses submitted by these authors in partial fulfillment of the requirements for the degree of Master of Science in Veterinary Medical Science.