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Inhibition of Macrophage Migration by a Skin-Reactive Polysaccharide from BCG Culture Filtrates

From the Laboratory of Bacterial Products, Division of Biologics Standards, National Institutes of Health, United States Department of Health, Education and Welfare, Bethesda, Maryland 20014

Abstract

A skin-reactive polysaccharide isolated from unheated BCG culture filtrates (GAE) inhibited guinea pig peritoneal exudate cell migration to the same degree as PPD in guinea pigs sensitized 2 to 3 months previously. The hydrolyzed polysaccharide and a second polysaccharide, both skin non-reactive, were inactive in this in vitro model of delayed hypersensitivity. The degree of migration inhibition correlated well with skin test size in recently sensitized (2 to 3 months) animals for both polysaccharide and protein antigens but bore no relationship to serum antibodies. With time, the ability of exudate cells from skin-reactive animals to react in vitro to the polysaccharide antigen disappeared while in vitro reactivity to PPD remained. We conclude that GAE, as well as a number of other polysaccharides, is able to induce and elicit delayed hypersensitivity.

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				S. D. Chaparas, D. E. Thor, H. P. Godfrey, H. Baer, and S. R. Hedrick Tuberculin-Active Carbohydrate That Induces Inhibition of Macrophage Migration but not Lymphocyte Transformation Science, November 6, 1970; 170(3958): 637 - 639. [Abstract] [PDF]