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Immunoglobulin Production in Cloned Sublines of a Human Lymphocytoid Cell Line¹

From the Departments of Biochemistry Research, Medicine C, and Surgery, Roswell Park Memorial Institute, ³ Buffalo, New York 14203

Abstract

In the present study we obtained seven sublines (five diploid and two tetraploid) by cloning LK1D cell line and found that all the sublines contained individual cells which synthesized both and heavy chains along with a single type of light chains, kappa chains. Six of these sublines showed homogeneous karyotypes which were characteristic of each subline, indicating that each subline was most likely to have been derived from a single ancestor cell. Our results show that cells which do not react with anti-immunoglobulin antibodies at a particular time of harvest have the potential to produce immunoglobulins. Moreover, two different heavy chains detected in individual cells are actually synthesized in the same cell and are not due to the transfer of immunoglobulins among the cells.

Footnotes

This work was supported in part by Grant AI-08472 from the National Institute of Allergy and Infectious Diseases.

² Present address: Department of Zoology, Faculty of Science, Hokkaido University, Sapporo, Japan.

³ A unit of the New York State Department of Health.

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