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Availability of Transplantation Alloantigens to Circulating Antibodies: Studies with Alloantibodies of Known Specificity¹

From the Departments of Biochemistry Research and Experimental Biology, Roswell Park Memorial Institute, ³ Buffalo, New York 14203

Abstract

Purified radiolabeled alloantibodies bind specifically to transplantation antigens in vivo following intravenous injection. The antibodies are localized in decreasing amounts by liver, lung, skin, spleen, kidney and brain. However, the ratio between specific and background, i.e., nonspecific, localization of radiolabeled globulins is greatest in the spleen. This reflects a higher concentration of alloantigens on the surface of spleen cells.

Antibodies directed against antigens specified either by the H-2 locus or by other H loci localize with comparable distribution patterns in separate assays in vivo. Localization is measurable even when the reaction of alloantibodies is restricted to single antigens of the H-2 complex.

The results indicate that transplantation antigens are available to humoral alloantibodies in mice and suggest that the paired radiolabeled technique may be used for identifying cells in recipients of foreign grafts and for histocompatibility typing.

Footnotes

This work was supported in part by Contract AT-(30-1)-2651 from the United States Atomic Energy Commission and Grant T-476 of the American Cancer Society.

² Present address: Department of Microbiology, Tel-Aviv University, Tel-Aviv, Israel.

³ Unit of New York State Department of Health.