HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via Google Scholar Google Scholar Articles by Romeyn, J. A. Articles by Ross, S. Search for Related Content PubMed Articles by Romeyn, J. A. Articles by Ross, S.

Anti-Immunoglobulin Control of Immune Hemolysis¹

From the Department of Medical Microbiology, University of Manitoba, Winnipeg, Manitoba

Abstract

19 S anti-immunoglobulin inhibits complementary lysis of erythrocytes sensitized with 7 S or 19 S hemolysin.

7 S anti-immunoglobulin inhibits complementary lysis of erythrocytes sensitized with 19 S hemolysin but facilitates lysis of erythrocytes sensitized with 7 S hemolysin.

7 S anti-immunoglobulin prepared by immunizing guinea pigs with normal rabbit serum does not facilitate lysis by 7 S hemolysin, whereas 7 S anti-immunoglobulin made by immunizing with guinea pig erythrocytes coated with rabbit hemolysin does facilitate such hemolysis.

7 S and 19 S autologous anti-immunoglobulin are present in rabbit-produced anti-erythrocyte serum in amounts which can substantially influence the lytic power of the serum or its fractions. Thus, the presence of such anti-immunoglobulin acts as a mechanism controlling the effectiveness of cytolytic antibody, presumably in vivo as well as in vitro.

Mercaptoethanol destroys the lytic capacity of the 7 S fraction of anti-erythrocyte serum and this can be restored to its original level by anti-immunoglobulin. In addition, anti-immunoglobulin can facilitate the lytic capacity to as high a level as that achievable with untreated 7 S hemolysin.

Footnotes

This work was supported by the Medical Research Council of Canada, under MRC Grant MA-1261, and by the University of Manitoba.