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A Method for the Immunoadsorption of Cells to an Antibodycoated Polyurethane Foam

From the Laboratory of Biochemistry, National Cancer Institute, National Institutes of Health, Department of Health, Education, and Welfare, Bethesda, Maryland 20014

Abstract

Reticulated polyester polyurethane foam was found to be a suitable matrix for the immunoadsorption of cells. The column used to support the foam was mounted in a horizontal position and slowly rotated about its long axis in order to permit maximal contact between cells and the foam surface while at the same time allowing free passage of unbound cells through the column. Bound cells were easily removed, intact, by gently squeezing the foam.

Untreated foam had a high affinity for guinea pig erythrocytes suspended in physiologic medium at pH 7.4. This nonspecific affinity was greatly reduced by pretreating the foam with various polyanions. Immunospecific binding of erythrocytes was achieved by adding anti-erythrocyte antibody to a gum arabic solution used to protect the foam. A 38-fold increase in binding of the cells was thus obtained. A single antibody-coated foam disk having a diameter of 1 in. and a thickness of in. was capable of binding up to 3 x 10⁸ guinea pig erythrocytes. The high specificity of this method was further demonstrated by the finding that erythrocytes coated with haptens were bound specifically to foam coated with their corresponding anti-hapten antibodies, with a low background of cross-reactivity.