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Histamine Release from Human Leukocytes by Anti-E Antibodies¹

From the Children's Asthma Research Institute and Hospital, Denver, Colorado, and the University Hospital and Institute of biochemistry, University of Uppsala, Uppsala, Sweden

Abstract

Rabbit antibodies specific for human E released histamine from the leukocytes of atopic patients, whereas antibodies specific for the other immunoglobulin classes did not. Antibodies specific for the light chains of immunoglobulins also released histamine. The results indicated that the histamine release is a reversed type allergic reaction. The concentration of anti-E required for 50% histamine release from leukocytes varied, depending on the donors. This concentration roughly correlated with the minimal concentration of the same antibody required to induce erythema-wheal reactions in their skin but was unrelated to the E concentration in their sera. The sensitivity of leukocytes to anti-E was increased by passive sensitization of the cells with reaginic serum containing a high concentration of E. The results showed that E is present on/in leukocytes and that E antibodies on the cells are responsible for histamine release by allergen.

Footnotes

This work was supported in part by Research Grant AI-04985 from the United States Public Health Service. Some of the results described in this paper were reported at the annual meeting of the American Association of Immunologists, April, 1968.