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Do Antibody-Forming Cells Circulate in the Blood?¹

From The Department of Pathology, The University of Chicago, Pritzker School of Medicine, Chicago, Illinois

Abstract

Criteria have been presented for differentiating hemolytic plaques produced by rat cells into three categories. These categories are determined by the effect on plaque formation of rotenone, a metabolic inhibitor, and papain, a sulfhydryl protease. The first in these categories, "true" plaques, are formed by the release of intracellular antibody from cells requiring an intact cellular respiratory system for such release. The second group, "passive release" plaques, are produced by intracellular antibody from cells which can release this antibody in the presence of metabolic inhibitors. The third, "false" plaques, are formed by antibody which was adherent to cell-platelet aggregates.

Spleen cells from recently immunized rats produced either "true" or "passive release" plaques. All plaques produced by peripheral blood cells appeared to be "false" plaques.

Footnotes

This research was supported by United States Public Health Service Grants AI-4197-07 and HE-05667.

² Supported by USPHS Training Grant AI-96.

³ Supported by USPHS Training Grant GM-0093.