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Quantitative Estimation of Antibodies in Different Immunoglobulin Classes¹

From the Department of Immunology, Middlesex Hospital Medical School, London, W.1., U.K.

Abstract

Human thyroglobulin autoantibodies in each major Ig class can be estimated quantitatively by adsorption onto insoluble antigen followed by dissociation with acid and assay by single radial immunodiffusion using appropriate antisera. The antibodies were preponderantly IgG. The results correlated well with values for antigen-binding capacity determined by coprecipitation with specific anti-Ig, of complexes formed with labeled thyroglobulin. The method, which was previously used in a study of antiglobulin factors in sera of patients with rheumatoid arthritis, can be applied to other antigen-antibody systems and to the determination of antibody in immunoglobulin sub-classes.

Footnotes

The work was supported by grants from the Medical Research Council, the Arthritis and Rheumatism Council, the Nuffield Foundation and the World Health Organization.