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A Study of the Antigenicity of Formaldehyde- and Glutaraldehyde-Treated Bovine Serum Albumin and Ovalbumin-Bovine Serum Albumin Conjugate

From the Biochemistry Department, St, Jude Children's Research Hospital and Department of Microbiology, University of Tennessee Medical Units, Memphis, Tennessee 38101

Abstract

Chemical, conformational and antigenic studies of bovine serum albumin (BSA) treated with formaldehyde or glutaraldehyde, as well as a conjugate formed by intermolecular cross-linking of ovalbumin (OA) to BSA, were undertaken. Formaldehyde reacted predominantly with the free amino groups and caused intramolecular cross-links with no apparent change in shape or antigenicity of the molecule. Glutaraldehyde caused intermolecular cross-linkages which formed soluble aggregates; such modified proteins were antigenic in rabbits and produced antibodies with two specificities, one directed against antigenic determinants on BSA and the other against newly acquired groups arising from the modification. Anti-OA-BSA conjugate contained antibodies against antigenic determinants of BSA, OA, and glutaraldehyde-treated BSA and OA.

Footnotes

¹ Present address: Division of Rheumatology and Department of Biochemistry, University of Alabama Medical Center, Birmingham, Alabama 35233.

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