HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via Google Scholar Google Scholar Articles by Golub, E. S. Articles by Weigle, W. O. Search for Related Content PubMed Articles by Golub, E. S. Articles by Weigle, W. O.

Studies on the Induction of Immunologic Unresponsiveness

III. Antigen Form and Mouse Strain Variation¹

From the Department of Experimental Pathology, Scripps Clinic and Research Foundation, La Jolla, California 92037

Abstract

The variation in the dose of ultracentrifuged human globulin (HGG) needed to produce an unresponsive state in different strains of inbred mice was shown to be the result of differences in the efficiency with which these strains process the trace amount of aggregated material remaining. Although mice from both C57BL/6J and BALB/cJ strains remove the aggregated material from the vascular fluids following injection of HGG, the BALB/cJ mice appear to process this material efficiently, and an immune response rather than an unresponsive state results. Conversely, C57BL/6J mice appear to process the aggregated material inefficiently, thus permitting the induction of unresponsiveness to the non-aggregated (tolerogenic) material. When the trace amount of aggregates was removed by salt fractionation, mice from both strains became unresponsive to small doses of HGG. The tolerogenic, deaggregated HGG was rendered immunogenic by chemical aggregation. The dose of HGG to which mice become unresponsive appears to be controlled by a genetic mechanism involving more than one gene.

C57BL/6J mice readily became unresponsive following injections of deaggregated G isolated from normal rabbit serum, but made an antibody response to G (ALG) isolated from the sera of rabbits immunized with mouse thymus cells. Mice made unresponsive to deaggregated RG did not lose their unresponsive state following injection of ALG.

Within the limits of the test system that was used, no low-dose unresponsive state was observed in C57BL/6J and BALB/cJ mice injected with ultracentrifuged HGG.

Footnotes

Publication 299 from the Department of Experimental Pathology, Scripps Clinic and Research Foundation, La Jolla, California. The work was supported by United States Public Health Service Grant AI-07007 and Atomic Energy Commission Contract AT (04-3)-410.

² Supported by United States Public Health Service Training Grant GM-683. Present address: Department of Biological Sciences, Purdue University, Lafayette, Indiana.

³ This work was supported by United States Public Health Service Research Career Award K6-GM-6936.