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Mechanism of Immunologic Tolerance

I. Induction of Tolerance to Bovine -Globulin by Injection of Antigen Into Intact Organs in Vitro¹

From the Department of Microbiology, Yale University, New Haven, Connecticut 06510

Abstract

Specific unresponsiveness to BG was demonstrated in thymectomized, irradiated Lewis rats restored with washed cells from intact lymphoid organs injected with soluble BG and incubated for 2 hr in vitro. Both delayed sensitization and antibody formation against BG were reduced in the recipient; responses to PPD and bovine A-globulin were not affected. Unresponsiveness to soluble ovalbumin could be induced by use of the same technique. Evidence is presented that this phenomenon is not due to the transfer of either free antigen, with the production of low-zone tolerance, or of cell-associated (membrane-bound) antigen. Since comparable unresponsiveness was not induced by incubating lymphoid cells in suspension with BG, it is suggested that a processing step may be necessary for the production of tolerance or, alternatively, that the physiologic environment of the lymphoid cells controls their reactivity with antigen.

Footnotes

These studies were supported by United States Public Health Service Grants AI 06112 and AI 06455. These data were included in a thesis submitted to the Graduate School of Yale University in partial fulfillment of the degree of Doctor of Philosophy.

² Supported by United States Public Health Service Training Grant AI 00291.