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Stimulation of Immunoglobulin and Anamnestic Antibody in Vitro¹

From the Department of Bacteriology and Immunology, Harvard Medical School, Boston, Massachusetts

Abstract

Fragments of lymph node from previously immunized rabbits were secondarily stimulated in vitro and placed under tissue culture conditions with the addition of ¹⁴C-leucine to the culture media. Antibody synthesis as determined by incorporation of leucine began earlier than that detectable by passive hemagglutination. It was found that in such cultures the secondary antibody response was superimposed on a steady synthesis of immunoglobulin, possibly antibody directed against other antigens.

Footnotes

The study was supported by United States Public Health Service Grant AI-05691.

² This work was carried out while a Predoctoral Fellow of the United States Public Health Service. It constitutes partial fulfillment of the requirements for the Ph.D. degree in the Division of Medical Sciences, Harvard University.

³ Present address: Department of Immunology, Hebrew University-Hadassah Medical School, Jerusalem, Israel.

⁴ Career Investigator, American Heart Association.