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The Journal of Immunology, 1969, 102: 292-297.
Copyright © 1969 by The American Association of Immunologists, Inc.
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Humoral Immunity in Rodent Malaria1

I. Estimation of Parasitemia by Electronic Particle Counting

Carter L. Diggs2 and Abraham G. Osler3

From the Department of Microbiology, The Johns Hopkins University, Baltimore, Maryland

Abstract

Parasitemia in young rats infected with Plasmodium berghei can be monitored by electronic counting of particles in blood lysates. The number of particles so obtained provides an estimate of the number of parasitized cells in the blood sample. The method is highly reproducible as compared to conventional techniques, especially as applied to low levels of parasitemia. The further advantage of rapidity is also inherent in the procedure.

Footnotes

1 Support for this investigation was provided, in part, by the National Science Foundation, Grant GB-1120; the American Cancer Society, Inc., Grant T-257; the National Institute of Allergy and Infectious Diseases, United States Public Health Service, Grant AI-03151; and the Office of The Surgeon General, Department of the Army, under the auspices of the Commission on Immunization, Armed Forces Epidemiological Board, Contract DA-49-193-MD-2468.

Presented in part at the Annual Meeting of the American Association of Immunologists, Atlantic City (Fed. Proc. 27: 369, 1968).

2 Portion of a dissertation submitted in partial fulfillment of the requirements for the Ph.D. degree. Present address: Chief, Department of Parasitology, USA Medical Component, SEATO, APO, San Francisco, Calif., 96346.

3 Present address: Department of Medical Immunology, The Public Health Research Institute of the City of New York, Inc., New York, N. Y. 10016, to which reprint requests should be addressed.






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