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The Journal of Immunology, 1969, 102: 273-282.
Copyright © 1969 by The American Association of Immunologists, Inc.

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Studies on Human Antibodies

VII. Acrylamide Gel Electrophoresis of Purified Human Antibodies and Myeloma Proteins, Their Heavy and Light Chains1

Marianne M. Dorner, William J. Yount and Elvin A. Kabat

From the Departments of Microbiology and Neurology, College of Physicians and Surgeons, Columbia University, the Neurological Institute, Presbyterian Hospital, and The Rockefeller University, New York, New York

Abstract

Six antibodies produced in one individual and anti-dextran formed in different individuals were compared with myeloma globulins in acrylamide gel electrophoresis. They were examined in their intact form as well as after reduction and alkylation. The findings indicate that antibodies and myeloma globulins represent fairly highly selected populations relative to normal {gamma}G immunoglobulin, with respect to their banding in acrylamide gel.

Footnotes

1 Aided by a grant from the National Science Foundation (GB-3675), the General Research Support Grant of the United States Public Health Service and Public Health Service Grant AM 09792-03.







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