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Protein-Carbohydrate Interaction

XVII. The Effect of Polysaccharide Molecular Weight on the Concanavalin A-Polysaccharide Precipitation Reaction¹

From the Department of Biological Chemistry, The University of Michigan, Ann Arbor, Michigan 48104

Abstract

Two-dimensional agar gel diffusion and quantitative precipitation studies of concanavalin A with several polysaccharides of varying molecular weights (dextrans, levans and glycogens) demonstrated the effect of polysaccharide molecular size on the concanavalin A-polysaccharide precipitation reaction. The effect of the molecular weight of these polysaccharides was manifested by their band positions in agar gel diffusion tests, i.e., the lower molecular weight species formed precipitation bands closer to the concanavalin A well.

The influence of polysaccharide molecular weight is evident also from quantitative precipitation studies, especially in the region of excess concanavalin A and of excess polysaccharide. Equivalence (or maximal precipitation) was reached earlier with the lower molecular weight polysaccharides; the lower molecular weight polysaccharides were also more effective in inhibiting precipitation in the region of polysaccharide excess.

Footnotes

This work was supported by United States Public Health Service Grant AM-10171, and a grant from the American Heart Association (67788).

² This work represents a portion of a doctoral dissertation submitted by Lucy L. So to the State University of New York at Buffalo.

³ Established Investigator of the American Heart Association.