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Water Soluble Guinea Pig Transplantation Antigen from Carcinogen-Induced Sarcomas

From The Laboratory of Immunology, National Institute of Allergy and Infectious Diseases, and the Surgery Branch, National Cancer Institute, Bethesda, Maryland

Abstract

Transplantation of carcinogen-induced sarcomas to allogeneic guinea pigs elicited a state of specific sensitization, such that a) donor-type skin grafts were rejected in accelerated fashion, and b) antigenic extracts prepared from donor-type normal tissues elicited cutaneous hypersensitivity reactions upon intradermal challenge. Water soluble transplantation antigen was liberated by exposure of dispersed tumor cells to low intensity sonic energy. Following gel filtration on Sephadex G 200 and discontinuous electrophoresis on polyacrylamide gel, the antigenic activity, as demonstrated by the elicitation of cutaneous hypersensitivity reactions in immunized hosts, was confined to a complex electrophoretic region at R_f 0.73–0.74, the same zone that possessed the antigenic activity of extracts prepared from normal tissues.

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				B. D. Kahan and R. A. Reisfeld Transplantation Antigens Science, May 2, 1969; 164(3879): 514 - 521. [PDF]