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The Journal of Immunology, 1969, 102: 266-268.
Copyright © 1969 by The American Association of Immunologists, Inc.
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Induction of Tolerance in Immunologically Competent Primates1

Richard J. Howard, John C. Landon2, Suanne F. Dougherty, Abner L. Notkins and Stephan E. Mergenhagen

From the National Institute of Dental Research, National Institutes of Health, Bethesda, Maryland 20014, and Bionetics Research Laboratories, Inc., a Division of Litton Industries, Kensington, Maryland 20795

Abstract

Immunologic tolerance has been induced in mice, rats, guinea pigs, rabbits and chickens with protein and polysaccharide antigens (1, 2). In addition, tolerance has been produced in fetal rhesus monkeys with large doses of bovine {gamma} globulin and in newborn humans with blood group antigens (3, 4). However, the induction of immunologic tolerance has not been reported in older primates. We report here the induction of tolerance to human {gamma} globulin (HGG) in immunologically competent monkeys.

Six-month- and one-year-old rhesus monkeys (Macaca mulatta) were bred and maintained at Bionetric Research Laboratories, Inc., Kensington, Maryland. In the first experiment, 6-month-old monkeys weighing 1500 to 2000 g were used. Animals were treated in a manner similar to that used to induce tolerance in adult mice (5, 6). Ten milligrams of HGG/ml (Cohn Fraction II, Hyland Laboratories, Costa Mesa, Calif.) in pyrogen-free saline was centrifuged for 2 hr at 105,000 x G in a Spinco model L-2 ultracentrifuge to remove aggregated proteins.

Footnotes

1 Portions of this study were conducted under Public Health Service Contract PH43-67-661 within the Special Virus Leukemia Program of the National Cancer Institute.

2 Bionetics Research Laboratories, Inc., a Division of Litton Industries, Kensington, Maryland 20795.






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