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The Journal of Immunology, 1969, 102: 253-262.
Copyright © 1969 by The American Association of Immunologists, Inc.
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Immunoglobulins of the Rhesus Monkey (Macaca Mulatta)

II. Properties of Rhesus IgG and IgM Antibody in Hemagglutination, Precipitation and Skin Sensitization1

James D. Lakin2, Roy Patterson3 and Jacob J. Pruzansky

From the Departments of Microbiology and Medicine, Northwestern University School of Medicine, Chicago, Illinois

Abstract

Separation of rhesus monkey IgG and IgM anti-bovine serum albumin (BSA) antibody from other immunoglobulin components of hyperimmune whole serum was accomplished by a combination of ammonium sulfate precipitation, diethylaminoethyl cellulose and Sephadex G 200 chromatography. Neither the IgG nor the IgM antibody preparations sensitized normal rhesus monkey skin for Prausnitz-Küstner type passive cutaneous transfer reactions. However, IgG anti-BSA antibody was shown to sensitize guinea pig skin for passive cutaneous anaphylaxis whereas IgM antibody of equal antigen-binding capacity did not. The relative efficiency of rhesus IgM anti-BSA in tanned red blood cell hemagglutination was found to be more than 23 times greater than that of an equal amount of IgG, as measured by BSA-binding capacity. In contrast, the ability of IgM to participiate in the precipitin reaction with soluble BSA was found to be poor in comparison to that of IgG antibody.

Footnotes

This study was supported in part by the Ernest S. Bazley Asthma Research Fund to Chicago Wesley Memorial Hospital, Chicago, Illinois.

2 Medical Scientist Training Program Awardee United States Public Health Service No. 5-T5-GM01671-03.

3 Ernest S. Bazley Professor of Allergy and Immunology.






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