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Antibody Activity Associated with the Fluorescent Polypeptide Chains of Human Immunoglobulin G¹

From the Department of Microbiology, The University of Virginia, Charlottesville, Virginia

Abstract

Direct immunofluorescent staining has been demonstrated with a conjugated human antistreptococcal IgG and its and light fluorescent polypeptide chains in 0.05 M sodium phosphate-0.02% sodium dodecyl sulfate, pH 7.5.

The activities of the IgG and its isolated and light chains are immunologically specific as indicated by the markedly greater inhibition of immunofluorescent staining of the streptococci by the unlabeled autologous IgG than by either homologous or heterologous inhibition with anti-staphylococcal serum or IgG.

The chains retain 12% while the light chains retain only 0.3% of the activity of the intact IgG, as judged by comparison of the intensity of immunofluorescence with the protein concentrations of the fluorescent IgG and the polypeptide chains.

Footnotes

This study was supported by United States Public Health Service Grants R01 CA 03726 and 5T01 AI 102.

² Fellow of the Life Insurance Medical Research Fund.

³ Recipient of a United States Public Health Service Research Career Development Award.