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Abstracts of Papers Presented at the Third International Complement Workshop, Harvard Medical School, Boston, Massachusetts, June 3–5, 1968

Synthesis of the First Component of Human Complement (C'1^Hu), In Vitro

National Cancer Institute, Bethesda, Md.

Abstract

We have presented evidence that the first component of guinea pig complement (C'1^gp) is synthesized by columnar epithelial cells of the small intestine. We now report that isolated segments of human colon and to a lesser extent ileum are capable of synthesizing hemolytically active C'1^hu. This conclusion is based on the following evidence: After elimination of C'1^hu from tissue with EDTA, we found that segments of the intestinal tract in short-term organ culture showed a 100- to 1000-fold increase in C'1^hu activity. The rate of production of C'1^hu in human intestine was highly temperature dependent; C'1^hu production was reversibly inhibited by puromycin and actinomycin D. Furthermore, ¹⁴C-labeled amino acids were incorporated into molecules which behaved like C'1. No significant C'1^hu synthesis was observed in isolated segments of jejunum, stomach, liver, kidney, lung, spleen or lymph node.