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The Application of the Irradiated Hamster Test to the Study of Experimental Allergic Encephalomyelitis¹

Department of Medical Genetics, University of Pennsylvania, Philadelphia, Pennsylvania

Abstract

Lymph node cells taken from rats sensitized to guinea pig spinal cord produced delayed skin reactions when mixed with antigen and injected into irradiated hamsters. Node cells from normal unsensitized rats failed to elicit such lesions. The immunologic basis for the skin reactions was demonstrated by their specificity for the inducing antigen, the delay in appearance of responsiveness in the lymph node cells until 2 weeks following immunization of the donor (a time period corresponding to the lag prior to clinical symptoms in the donor), and finally by the failure of node cells taken from donors rendered neonatally tolerant to spinal cord to react when challenged with the antigen. It was shown in addition that sensitized lymphoid cells from a strain of rat genetically insusceptible to the expression of clinical EAE also failed to react against the antigen in the irradiated hamster test. This may reflect a deficiency in these cells of a chemical mediator of delayed hypersensitivity.

Footnotes

This investigation was supported in part by Special Fellowship 1-F3-A1-20,465-01 and by Grant AI-07001 from the National Institutes of Health, Bethesda, Maryland.

² Present address: Department of Medicine, Hahnemann Medical College and Hospital, Philadelphia, Pennsylvania 19102.

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				D. L. Gasser, C. M. Newlin, J. Palm, and N. K. Gonatas Genetic Control of Susceptibility to Experimental Allergic Encephalomyelitis in Rats Science, August 31, 1973; 181(4102): 872 - 873. [Abstract] [PDF]