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Ontogeny of the Immune Response

V. Further Characterization of 19 S G- and 7 S G-Immunoglobulins in the True Primary Immune Response in Germfree, Colostrum-Deprived Piglets¹

From the Department of Microbiology, University of Minnesota, Minneapolis, Minnesota 55455

Abstract

Germfree, immunologically virgin piglets were used to study the ontogenic development of immunoglobulins. In the true primary immune response, the first antibody synthesized was 19 S G-immunoglobulin which was antigenically identical to the late immune 7 S G-immunoglobulin but distinct from 19 S M. In addition, there was a sequential synthesis of 7 S G-immunoglobulin before the appearance of M or A. It was, therefore, possible to isolate 19 S G free from 19 S M-macroglobulin and 7 S G free from A.

The purified 19 S G-immunoglobulin of the true primary immune piglet sera had a sedimentation constant of 18.5 S (s⁰_20,w) and a molecular weight of 900,000. The purified 7 S G-immunoglobulin of piglet and sow had a sedimentation constant of 6.6 S (s⁰_20,w) and a molecular weight of 170,000.

The specific antibody activity was demonstrated only in 19 S G- and 7 S G-immunoglobulins but not in 19 S M as determined by radio-immunoelectrophoretic technique using I¹³¹-labeled actinophage and keyhole limpet hemocyanin.

Evidence presented indicates that the 19 S G is not an artificial aggregate of 7 S G but is synthesized as a true immunoglobulin of the 19 S class.

Because of the physiochemical similarities of 19 S G and 19 S M, the difficulties involved in their separation and characterization when both are present in the sera are discussed.

Footnotes

This study was supported by United States Public Health Service Grant AI-03439 from the National Institute of Allergy and Infectious Diseases.