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Phagocytosis and Intracellular Fate of Mycobacterium Tuberculosis: In Vitro Studies with Guinea Pig Peritoneal and Alveolar Mononuclear Phagocytes¹

From the Veterans Administration Hospital, Microbiology Research³, and the Department of Microbiology, University of Utah, College of Medicine, ^,4 Salt Lake City, Utah

Abstract

The role of cellular immunity in tuberculosis was studied by using in vitro techniques to observe cellular capacities for phagocytosis and bacterial inactivation. Peritoneal exudates were stimulated and collected and alveolar phagocytes were harvested using standard techniques.

Normal and BCG-immunized guinea pigs were used as phagocyte donors. Two strains (H37Rv, H37Ra) of Mycobacterium tuberculosis were used for infecting the phagocytes. Suspensions of these tubercle bacilli consisting of single cells were prepared by filtration.

Plate counts were used to indicate the amount of ingestion and cytopepsis by the phagocytes. It was shown using the H37Rv strain of tubercle bacilli that phagocytes from BCG-immunized animals had increased cytopeptic activity when compared to phagocytes from normal animals. Also, the alveolar phagocytes were more active in cytopepsis than were the peritoneal exudate cells.

Footnotes

This work was presented in part at the Annual Meeting of the American Society for Microbiology (Bact. Proc., p. 93, 1967) and was supported by research grants from the National Institutes of Health (A1-K6-14,924; CA 07302).

The experimental animals used in these studies were fed, housed, and cared for in a humane manner and such care was supervised by a member of the Animal Care Panel.

² The material in this report is taken from a thesis submitted by Kameron W. Maxwell in partial fulfillment of the requirements for the degree of Doctor of Philosophy, June 1966.

³ Kameron W. Maxwell.

⁴ Stanley Marcus.