HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Chuba, J. V. Articles by Nigrelli, R. F. Search for Related Content PubMed Articles by Chuba, J. V. Articles by Nigrelli, R. F.

The Use of Catfish, Ictalurus Nebulosus (Le Sueur), as Experimental Animals for Immunization with Human Secretor Saliva and Other Antigenic Materials¹

From the Department of Pathology, New York University School of Medicine and the Osborn Laboratories of Marine Sciences, New York Aquarium, New York Zoological Society, Brooklyn, New York

Abstract

Five of eight brown bullhead catfish surviving a 6-week series of three immunizing injections of human secretor saliva, mixed with Freund's complete adjuvant, produced high titered heteroagglutinins against human erythrocytes of all A-B-O types. Agglutination-inhibition and cell absorption tests indicated potent fractions of activity specific for human A, B, or O erythrocytes corresponding to the A-B-O group of the secretor saliva injected.

Bullfrog erythrocytes with B-like specificity were agglutinated only by the hyperimmune plasma from the catfish injected with group B saliva. Sheep erythrocytes were weakly hemolyzed but not agglutinated by the catfish plasma specimens.

The usefulness of the brown bullhead catfish, both from the point of view of the quantities of blood obtainable by repeated bleedings, as well as the immunologic competence of the species to produce specific high titered antibodies in response to injected antigenic materials of major interest in human blood group studies, has been clearly established.

Footnotes

This work was supported by United States Public Health Service training Grant AI 00247-05 and The Rockefeller Foundation Grant (RF 64078) to New York Zoological Society.

This article has been cited by other articles:

				C. Grossman Interactions between the gonadal steroids and the immune system Science, January 18, 1985; 227(4684): 257 - 261. [Abstract] [PDF]