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Communications

Heparin-MnCl₂ Removal of Nonspecific Serum Inhibitor for Measles Virus Hemagglutinin¹

From the Department of Preventive Medicine, State University of New York, Upstate Medical Center, Syracuse, New York

Abstract

Recently we demonstrated (1) that heparin-MnCl₂ removes nonspecific rubella hemagglutinin inhibitor more effectively from serum than does kaolin. Mann et al. (2) previously had reported that the heparin-MnCl₂ combination was superior to kaolin in precipitating the nonspecific serum inhibitor for reovirus hemagglutinin. Both of these nonspecific serum inhibitors are -lipoproteins (1, 2) which kaolin adsorbs irregularly. Heparin-MnCl₂ precipitates it uniformly. While kaolin may reduce the concentration of IgG substantially, heparin-MnCl₂ does not. Kaolin also introduces a dilution factor which can be difficult to estimate with confidence.

This report is concerned with the extension of the heparin-MnCl₂ system to the removal of the nonspecific measles virus hemagglutinin inhibitor (3) from human sera. As the data illustrate, this is accomplished as effectively as in the rubella system. In this investigation, the sera of 50 young children, which had been stored for several years at -20°C, were thawed and 1.0-ml amounts were removed from each.

Footnotes

¹ The author is indebted to Mrs. Carol Sloan and Mrs. Inara Rubulis for their technical assistance.