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Regulation of Antibody Synthesis Against Escherichia Coli Endotoxin

I. Suppressive Effect of Endogenously Produced and Passively Transferred Antibodies

From the Department of Tumor Biology, Karolinska Institutet Medical School, Stockholm 60, Sweden

Abstract

Cyclical fluctuations of cellular and humoral 19 S antibody synthesis were evoked by one single injection of heat-killed E. coli 055:B5 bacteria into CBA mice. The phenomenon was interpreted as involving feedback suppression of active antibody synthesis by 19 S antibodies. The biologically stable antigen would be prevented from stimulating antibody synthesis by reacting with the initially produced 19 S antibodies. In the absence of further stimulation 19 S-producing cells and serum antibodies would decay, leaving antigen free to initiate a second cycle of antibody synthesis. This hypothesis was supported by the finding that antiserum taken from the time of the first peak could suppress the appearance of a second peak.

Antigen and 19 S antibodies injected simultaneously delayed active antibody synthesis which eventually appeared spontaneously. Presumably this represents a more rapid catabolism of the blocking antibodies than of the antigen.

7 S antibodies were not detected against this antigen during the primary response but appeared after intense hyperimmunization. The most sensitive method for detection of 7 S antibodies was inhibition of antibody synthesis by passive transfer of purified 7 S fractions.

7 S antibodies were more potent in inhibiting active antibody synthesis than 19 S antibodies when equal quantities of hemagglutinins were injected.

Footnotes

¹ Present address: Department of Bacteriology, Karolinska Institutet Medical School, Stockholm 60, Sweden.

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